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Our data demonstrate that both the kinetic and mechanical properties of these two myosins, in addition to the stochiometry of NM-2 subunits, can tune filament mechanical output.

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For protein expression, bacteria was grown in M9 medium with 50 mg/L of kanamycin at 37 °C until OD600 ∼ 0.6, cooled down to 30 °C, induced with 0.1 mM IPTG and protein was overexpressed at 30 °C overnight.

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OEM TUNE vs CUSTOM TUNE. I often get the question: "What makes your engines / tune better than stock or your competition". Many "turnkey engine" suppliers 

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'Product' refers to the package that is uploaded to the GO Publish= er Admin Console, and contains configuration information for your specified= dataset, using the settings configured in your GO Publisher Desktop projec= t (.gpp).

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